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| Registros recuperados : 2 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
24/05/2021 |
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Data da última atualização: |
24/05/2021 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
PRADO, G. S.; PINHEIRO, T. T.; FARIA, J. C. de; VIANELLO, R. P. |
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Afiliação: |
GUILHERME SOUZA PRADO, especialista visitante CNPAF; THAÍSA TESSUTTI PINHEIRO, especialista visitante CNPAF; JOSIAS CORREA DE FARIA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Genome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP). |
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Título original: |
Tecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura, 2020. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021. |
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Páginas: |
p. 47-88. |
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Descrição Física: |
il. |
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ISBN: |
978-65-87380-10-0 |
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Idioma: |
Inglês |
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Conteúdo: |
We will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments. |
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Palavras-Chave: |
CRISPR; CRISPRevolution; Non-homologous. |
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Thesagro: |
Biotecnologia; DNA; Engenharia Genética; Genoma; RNA. |
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Thesaurus NAL: |
Biotechnology; Genetic engineering; Nucleases; Plant genetics; Ribonucleoproteins; RNA editing. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/223400/1/chap2.pdf
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Marc: |
LEADER 01908naa a2200361 a 4500
001 2131989
005 2021-05-24
008 2021 bl uuuu u00u1 u #d
020 $a978-65-87380-10-0
100 1 $aPRADO, G. S.
240 $aTecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura, 2020.
245 $aGenome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP).$h[electronic resource]
260 $c2021
300 $ap. 47-88.$cil.
520 $aWe will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments.
650 $aBiotechnology
650 $aGenetic engineering
650 $aNucleases
650 $aPlant genetics
650 $aRibonucleoproteins
650 $aRNA editing
650 $aBiotecnologia
650 $aDNA
650 $aEngenharia Genética
650 $aGenoma
650 $aRNA
653 $aCRISPR
653 $aCRISPRevolution
653 $aNon-homologous
700 1 $aPINHEIRO, T. T.
700 1 $aFARIA, J. C. de
700 1 $aVIANELLO, R. P.
773 $tIn: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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